产品中心
详询华雅旗下公司红荣微再 :1500 1904 520。红荣微再-客服: 经销商专员
Illumina Miseq测序仪测序必需试剂盒,包括簇生成、双端测序和边合成边测序试剂(SBS)。
Illumina- MiSeq Kit
产品英文名称:MiSeq Kit
产品中文名称:MiSeq Kit试剂盒
用途:小型全基因组测序(微生物、病毒),靶向基因测序(扩增子、基因panel),16S宏基因组测序,靶向基因表达谱分析,miRNA和Small RNA分析,长片段扩增子测序,DNA-蛋白质相互作用分析
货号:
MS-102-2001/MS-102-2002/MS-102-2003/MS-102-3001/MS-102-3003/MS-103-1001/MS-103-1002/MS-103-1003
MiSeq Kit介绍
要在 MiSeq 上执行运行,需要一个一次性 MiSeq 试剂盒。
该试剂盒有各种不同的类型和规格。每种类型的 MiSeq 试剂盒都包含一个试剂盒的流动槽类型和执行一次运行所需的全部试剂。试剂盒中提供的流动槽、PR2 瓶和试剂盒均使用射频识别(radio-frequency identification,简称 RFID)进行精确的耗材跟踪并确保兼容性。始终使用与您的流动槽类型相匹配的试剂盒。如果试剂盒不兼容,运行设置期间会显示一条消息,提示您装入兼容的试剂盒。
流动槽:flow cell
MiSeq Kit特点:
改良化学过程后,循环时间更快、读长(reads)更长、输出更多
双表面成像实现的读长数是v1试剂盒单表面成像的两倍
使用500次循环的试剂盒可扩展读长长度
选择您应用的循环次数(50、300或500)
借助MiSeq试剂盒v3,研究人员可:
使单次运行的输出翻倍
使用600次循环试剂盒,使reads长度zui长延伸至2 x 300碱基对
将reads数量增至2500万个,以开创新的应用
The MiSeq Reagent Kit is a single-use reagent kit required to perform a sequencing run. It is available in different types and sizes. Each type of MiSeq Reagent Kit includes a kit-specific flow cell type and all reagents required for performing a run.The flow cell, PR2 bottle, and reagent cartridge provided in the kit use radio-frequency identification (RFID) for accurate consumable tracking and compatibility.Always use the reagent cartridge associated with your flow cell type. If the reagent cartridge is not compatible, a message appears during run setup that prompts you to load a compatible reagent cartridge.
Get faster cycle times, longer reads and more output from improved chemistry
Dual surface imaging enables twice the number of reads compared to single surface imaging with the v1 kit
Extend your read lengths with the 500-cycle kit
Choose the perfect number of cycles for your application (50, 300, or 500)
MiSeq Reagent Kit v3 allows researchers to:
Double the output of a single run
Extend read lengths up to 2 x 300 bp with the 600-cycle kit
Increase the number of reads to 25 million to unlock new applications
MiSeq Kit应用
小型全基因组测序(微生物、病毒),靶向基因测序(扩增子、基因panel),16S宏基因组测序,靶向基因表达谱分析,miRNA和Small RNA分析,长片段扩增子测序,DNA-蛋白质相互作用分析
实例:
上游试剂 癌症panel:TruSeq Amplicon - Cancer Panel
Illumina MiSeq Kit 参数
zui高通量 | 8.5 Gb (500-cycle MiSeq Reagent Kit v2), 5.1 Gb (300-cycle MiSeq Reagent Kit v2), 1.2 Gb (300-cycle MiSeq Reagent Micro Kit v2), 0.850 Gb (50-cycle MiSeq Reagent Kit v2), 0.5 Gb (500-cycle MiSeq Reagent Nano Kit v2), 0.3 Gb (300-cycle MiSeq Reagent Nano Kit v2),15 Gb (600-cycle MiSeq Reagent Kit v3), 3.8 Gb (150-cycle MiSeq Reagent Kit v3) |
zui高读长 | Up to 15 million(v2),Up to 25 Million(v3) |
试剂类型 | Cluster Generation, Paired-End Sequencing, Sequencing by Synthesis |
测序仪 | MiSeq, MiSeq FGx in Research Mode, MiSeqDx in Research Mode |
组份 | 数量 | 描述 | 保存 |
Reagent Cartridge | 1 | Single-use pre-filled cartridge | -25°C to -15°C |
HT1 | 1 | 5 ml tube, Hybridization Buffer | -25°C to -15°C |
Flow Cell | 1 | Single-use flow cell | 2°C to 8°C |
PR2 | 1 | 500 ml bottle, Incorporation Buffer | 2°C to 8°C |
自备试剂:
备用的 1.0 N NaOH,分子生物学级
酒精擦拭纸,70%甘油或乙醇,70%
一次性无粉手套
低尘实验室棉巾纸
微量离心管
擦镜纸,4 x 6 英寸
MiSeq试管,Illumina
NaOCl,5%
Tween 20
实验室级用水
订购信息
品牌 | 货号 | 名称 | 规格 |
Illumina | MS-102-2001 | MiSeq Reagent Kit v2 | 50 cycles |
Illumina | MS-102-2002 | MiSeq Reagent Kit v2 | 300 cycles |
Illumina | MS-102-2003 | MiSeq Reagent Kit v2 | 500 cycles |
Illumina | MS-102-3001 | MiSeq Reagent Kit v3 | 150 cycles |
Illumina | MS-102-3003 | MiSeq Reagent Kit v3 | 600 cycles |
Illumina | MS-103-1001 | MiSeq Reagent Nano Kit v2 | 300 cycles |
Illumina | MS-103-1002 | MiSeq Reagent Micro Kit v2 | 300 cycles |
Illumina | MS-103-1003 | MiSeq Reagent Nano Kit v2 | 500 cycles |
注:micro 和 nano的区别在于测序通量。
Illumina- MiSeq Kit及更多测序产品详询
华雅再生医学旗舰公司:红荣微再(上海)生物工程技术有限公司 :1500 1904 520。红荣微再-客服: 经销商专员
红荣微再主营精准医疗和细胞治疗等产品。