人iPS诱导多能干细胞，搭配已驯化培养基

• 型   号：Cellartis
• 价   格：22718

人iPS诱导多能干细胞，搭配已驯化培养基，是一个完整的iPS诱导多能干细胞培养体系。
包括人iPS诱导多能干细胞和用于增殖培养的Cellartis DEF-CS Culture System培养体系。人皮肤细胞使用逆转录技术转入Oct-4，SOX2，KLF4和c-Myc，所得iPS细胞表达Oct-4，NANOG，SSEA-3，SSEA-4，TRA-1 -60，TRA-1-81干细胞特异性标志物。

订购留言

分享到：

 华雅旗下红荣微再授权代理（上海）TAKARA CellArtis，Rubicon产品 

1500 1904 520 红荣微再-客服: 经销商专员

产品图片仅供参考。实际产品包括细胞株+培养基+添加剂+包被剂

人iPS诱导多能干细胞

(Human Induced Pluripotent Stem Cells)

产品英文名称：Cellartis® Human iPS Cell Lines

产品中文名称：Cellartis® 人iPS诱导多能干细胞

Cellartis人iPS细胞（Human iPS Cell Line）产品是达到了工业客户使用标准生产的干细胞产品。该产品包括细胞和用于增殖培养的Cellartis DEF-CS Culture System培养体系。iPS细胞已经在制品化时完成了对DEF-CS培养体系的适应。Cellartis DEF-CS Culture System培养体系是组分明确而且不依赖饲养层的便捷的干细胞培养体系。Cellartis DEF-CS Culture System适合高效率地增殖培养干细胞，并且保持干细胞在增殖过程中几乎无分化地生长，这使得不需要在大规模增殖培养过程中进行细胞筛选。因此，Cellartis人iPS细胞（Human iPS Cell Line）产品是一套完整的产品体系，对于科研级别和工业规模的需求都适合。

Cellartis人iPS诱导多能干细胞特点

☆细胞系通过使用缺陷型多顺反子逆转录病毒（defective polycistronic retrovirus）技术重编程人皮肤成纤维细胞以转入Oct-4，SOX2，KLF4和c-Myc

☆细胞系的纯度和干细胞特征经过广泛分析，包括解冻后的恢复，支原体和细菌检测，干细胞特异性标记物的表达（Oct-4，NANOG，SSEA-3，SSEA-4，TRA-1 -60，TRA-1-81），不表达分化标记（β-3微管蛋白，FOXA2，ASMA）和预期核型

☆用Cellartis DEF-CS培养系统优化培养，试剂盒包括Cellartis DEF-CS 100培养系统

☆适用于所有主要应用; 细胞在分化时可以分化形成所有三个胚层的衍生物

Cell lines were created by reprogramming human skin fibroblasts using defective polycistronic retrovirus technology to deliver Oct-4, SOX2, KLF4, and c-Myc

Cell lines are extensively analyzed for purity and stem-cell characteristics, including recovery after thawing, absence of mycoplasma and bacteria, expression of stem cell-specific markers (Oct-4, NANOG, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81), absence of differentiation markers (Beta-3 tubulin, FOXA2, ASMA), and expected Karyotype

Optimized for culture with the Cellartis DEF-CS Culture System

Suitable for all major applications; cells can form derivatives of all three germ layers when differentiated

Kits include the Cellartis DEF-CS 100 Culture System

Cellartis 人iPS诱导多能干细胞参数

不同货号对应供体年龄性别冷冻代数和已确认分化能力

1500 1904 520。红荣微再-客服: 经销商专员

iPS诱导多能干细胞的应用

细胞治疗、药物监测和疾病模型

Cellartis 人iPS诱导多能干细胞组份

不同供体的iPS细胞系和DEF-CS 100*培养基

*培养基包含基础培养基、添加剂和包被剂。

保存：细胞 ≤ –150°C，添加剂 –20°C,基础培养基和包被剂4 °C。

A. Cellartis Human iPS Cell Lines

Cellartis human iPS cell line P11025 (Cat. No. Y00180, not sold separay; sold as a part of Cat.No. Y00185)

Cellartis human iPS cell line P11032 (Cat. No. Y00220, not sold separay; sold as a part of Cat.No. Y00225)

Cellartis human iPS cell line 4 (ChiPSC4) (Cat. No. Y00260, not sold separay; sold as a part of Cat. No. Y00265)

Cellartis human iPS cell line 7 (ChiPSC7) (Cat. No. Y00270, not sold separay; sold as a part of Cat. No. Y00275)

Cellartis human iPS cell line 12 (ChiPSC12) (Cat. No. Y00280, not sold separay; sold as a part of Cat. No. Y00285)

Cellartis human iPS cell line 18 (ChiPSC18) (Cat. No. Y00300, not sold separay; sold as a part of Cat. No. Y00305)

Cellartis human iPS cell line 21 (ChiPSC21) (Cat. No. Y00310, not sold separay; sold as a part of Cat. No. Y00315)

Cellartis human iPS cell line 22 (ChiPSC22) (Cat. No. Y00320, not sold separay; sold as a part of Cat. No. Y00325)

B. Cellartis DEF-CS 100 Culture System

· Cellartis DEF-CS 100 Culture System (Cat. No. Y30020, not sold separay; sold as a part of Cat.Nos. Y00185, Y00225, Y00265, Y00275, Y00285, Y00305, Y00305, and Y00315. A larger 500ml version of this product is sold as Cat. No. Y00310.)

o DEF-CS Basal Medium (100 ml)

o DEF-CS COAT-1 (for 100 ml) (800 µl)

o DEF-CS GF-1 (for 100 ml) (300 µl)

o DEF-CS GF-2 (for 100 ml) (100 µl)

o DEF-CS GF-3 (for 100 ml) (40 µl)

另行准备材料：PBS+TrypLE 选择酶+TC处理ps表面培养器皿

The following materials are required but not supplied:

· PBS Dulbecco's with Ca2+ & Mg2+ (D-PBS +/+)

· PBS Dulbecco's without Ca2+ or Mg2+ (D-PBS –/–)

· TrypLE Select Enzyme (1X), no phenol red

· Cell culture vessels, Tissue culture treated polystyrene surface

· General cell culture equipment used in cell culture laboratory

参考文献：

1. de Peppo GM, et al. Osteogenic potential of human mesenchymal stem cells and human embryonic stem cell-derived mesodermal progenitors: a tissue engineering perspective. Tissue Eng Part A.2010 Nov;16(11):3413-3426.

2. Both SK, et al. Differential bone-forming capacity of osteogenic cells from either embryonic stem cells or bone marrow-derived mesenchymal stem cells. J Tissue Eng Regen Med.2011 Mar;5(3):180-190.

3. de Peppo GM, et al. Free-form-fabricated commercially pure Ti and Ti6A14V Porous Scaffolds support the growth of human embryonic stem cell-derived mesodermal progenitors. ScientificWorldJournal. 2012;2012:646417.Epub 2012 Jan 4.

4. Ou Li, et al. Human embryonic stem cell-derived mesenchymal stroma cells(hES-MSCs) engraft in vivo and support hematopoiesis without suppression immune function: implications for off –the shelf ES-MSC therapies. PLOS ONE.2013; 8(1):e55319.

Cellartis 人iPS诱导多能干细胞订购信息

不同货号的区别：iPS细胞供体不同

关联产品

人iPS细胞*培养基

http://www.chem17.com/st321032/Product_29103798.html

华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司主营细胞治疗和精准医疗等产品，2018年与TAKARA继续合作，授权代理（上海）其旗下Cellartis干细胞研究制品和Rubicon单细胞文库构建试剂。Cellartis公司位于瑞典，拥有iPS细胞等干细胞分化为肝细胞及脏器细胞的分化诱导技术和ES细胞，ips细胞，分化细胞等干细胞相关产品。

手机1500 1904 520 红荣微再-客服  经销商专员