BioLife Solutions GMP级CryoStor CS5 Freeze Media细胞DMSO冻存液
产品英文名 BioLife Solutions CryoStor CS5 Freeze Media
产品中文名 BioLife Solutions CryoStor CS5细胞DMSO冻存液
产品介绍 BioLife Solutions CryoStor®是BioLife公司特别优化的细胞冻存液,在极低温度 (-70ºC to -196ºC)下准备和保存细胞。预混DMSO,为细胞和组织的冷冻、储存和解冻过程提供安全的保护环境。CryoStor®通过调控冻存过程的分子生物学反应,无血清、蛋白或具有高细胞毒性的试剂,就能增强细胞活力和功能。
产品订购信息
品牌 | 货号 | 产品描述 | 包装 |
BioLife Solutions | 205373 | CryoStor CS5 Freeze Media 细胞DMSO冻存液 | 5×10mL |
BioLife Solutions | 205102 | CryoStor CS5 Freeze Media 细胞DMSO冻存液 | 100 mL/瓶 |
BioLife Solutions | 205202 | CryoStor CS5 Freeze Media 细胞DMSO冻存液 | 100 mL/袋 |
CryoStor CS5细胞冻存液特点
用于细胞和组织的高级生物保存液
预混DMSO
无血清、无蛋白
USP级高质量成分、cGMP级生产、FDA master文件
灭菌、内毒素和细胞功能检测
DMSO无血清细胞冻存液适用细胞
多种类型细胞,包括如肝细胞之类的敏感细胞
BioLife Solutions CryoStor CS5 细胞DMSO冻存液cGMP级生产
MATERIALS ARE MANUFACTURED UNDER cGMP
CryoStor® Usage and Cryopreservation Protocol使用与冻存方法
细胞准备
1) Place cells to be cryopreserved into suspension (mechanical or enzymatic dissociation)
2) Centrifuge cells to obtain cell pellet
3) Remove supernatant - Note: Remove as much culture media as possible, to reduce dilution of CryoStor® solution.
加CryoStor冻存液
4) ISOLATION: Add cold (2-8°C) CryoStor®
a. Cell concentrations: 0.5-10 x 106cells/ml for routine cell culture protocols (higher [cell] possible).
b. DMSO is pre-mixed in CryoStor® - no additives are necessary.
5) PRE-FREEZE: Incubate cell suspension at 2-8°C for approximay 10 minutes
6) NUCLEATION: Freeze samples at -70°C (many protocols utlilize -70°C and -80°C interchangeably)
a. Use a controlled rate freeze (-1°C/min) or similar protocol for most mammalian cell systems.
b. The freezing device or isopropanol container should be pre-cooled to 2-8°C.
c. Ice nucleation within the sample (seeding) should be initiated at approximay -5°C using either a liquid nitrogen burst program setting on a controlled rate freezer or mechanical agitation (flick or tap) of the cryovial/sample container after approximay15-20 min. at -70°C.
d. Freeze time (-70°C) using isopropanol containers is recommended to be 3-4 hours.
储存
7) STORAGE: Place samples into storage
a. Store samples at liquid nitrogen temperatures (below -130°C).
b. Sample storage at -80°C is only recommended for short-term storage (weeks to months).
细胞复苏解冻
8) THAWING: Thaw samples quickly in a 37°C water bath
a. Sample thawing should be conducted with gentle swirling of sample until all visible ice has melted. Approximate thaw time for a 1 ml sample in a cryovial is approximay 3 minutes.
b. DO NOT allow sample to warm above chilled temperatures (0-10°C). Cryovials should be cool to the touch when removed from bath.Passive thaw is not recommended.
9) Dilute cell/CryoStor® mixture immediay with culture media
a. Dilution procedure can be preformed in a single step.
b. The dilution media should be between 20°C and 37°C.
c. A dilution ratio of 1:10 (sample to media) or greater is recommended.
10) Plate cells in appropriate configuration
11) Place cells into culture conditions or utilize immediay
12) Viability assessment 24-hours post-thaw*
Note: To obtain an accurate measure of cell viability following cryopreservation, assessment should be performed 24 hours post-thaw and compared to non-frozen controls.
*Sample assessment immediay post-thaw with membrane integrity indicators, such as Trypan Blue, for comparative analysis of sample cell yield and viability often results in significant overestimates of cell survival.
Live/Dead fluorescent assays or metabolic assays (MTT or alamarBlue®) are recommended for more accurate viability assessment.
Visual inspection of adherent cells and cells “floating” in the media is also recommended.
BioLife Solutions CryoStor CS5/CS10 Freeze Media 细胞冻存液
品牌 | 货号 | 产品描述 | 包装 |
BioLife Solutions | 210102 | CryoStor CS10 Freeze Media 细胞DMSO冻存液
| 100 mL/瓶 |
BioLife Solutions | 210210 | CryoStor CS10 Freeze Media 细胞DMSO冻存液 | 1000 mL/袋 |
BioLife Solutions | 210202 | CryoStor CS10 Freeze Media 细胞DMSO冻存液 | 100 mL/袋 |
BioLife Solutions | 210374 | CryoStor CS10 Freeze Media 细胞DMSO冻存液 | 16 mL/瓶 |
BioLife Solutions | 210373 | CryoStor CS10 Freeze Media 细胞DMSO冻存液 | 10 mL/瓶 |
BioLife Solutions | 205373 | CryoStor CS5 Freeze Media 细胞DMSO冻存液 | 5×10mL |
BioLife Solutions | 205102 | CryoStor CS5 Freeze Media 细胞DMSO冻存液 | 100 mL/瓶 |
BioLife Solutions | 205202 | CryoStor CS5 Freeze Media 细胞DMSO冻存液 | 100 mL/袋 |
CS5和CS10主要区别是规格包装不同。
BioLife Solutions CryoStor Freeze Media GMP级细胞冻存液现货供应欢迎您咨询华雅再生医学旗舰公司:红荣微再(上海)生物工程技术有限公司 :1500 1904 520。红荣微再-客服: 经销商专员