BD P100采血管
英文名称 BD P100 Blood Collection System，BD P100 collection tube
中文名称 BD P100 血浆蛋白保存系统，BD P100采血管
BD P100采血管含有EDTA和蛋白酶抑制剂，适合血浆蛋白质保存。其中8.5mL 规格（货号：366448）的BD P100采血管含有机械分离装置，离心后作为物理屏障隔开血浆和细胞。
华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司代理BD采血管系列。BD P100 血浆管（货号：366448 采血量：8.5mL）现货*（2018年5月）。
BD P100说明书
BD P100
用于血浆蛋白质保存的血液采集系统，科研级别
应用
BD P100 血液采集系统带有机械分离装置，提供了血浆采集、分离、运输和保存的手段，以便用于下游蛋白质分析。
该产品仅供科研使用，严禁使用本产品进行诊断程序和对象管理（subject management）。
体外诊断程序的临床使用和性能特征，BD P100采血管都没有建立。
产品描述
BD P100 采血管含有喷干的K2EDTA抗凝剂和适合的添加剂。
离心时，分离装置被激活，并移动到血浆和细胞层之间。分离结束后，血浆可以从BD P100采血管中直接吸出，免除转移血浆到第二个试管的步骤。
未提供的必需设备
采血针+持针器+防护
1.带有12英寸管道的血液采集装置（采血针），如BD Vacutainer® Safety-Lok™或BD Vacutainer® Push Button Blood Collection Set。
2.必须使用BD Vacutainer®持针器（Holder），例如BD Vacutainer® One Use Holder，以确保其功能发挥正常。
3.如果不使用仪器直接取样或者样品分开存放，则使用一次性移液器。
4.离心机要求：在管底部能够产生1100-3000g的离心力。
5.必要的手套，护目镜和其他个人防护装备，以防止接触血源性病原体。
采血配件（非说明书配图）
防止回流
BD P100采血管含有化学添加剂，与血液混合可能发生副反应，因此需要避免采血管内液体倒流人体。为防止回流，请遵守以下预防措施：
1.使用血液采集装置，例如BDVacutainer®Safety-Lok™或BD Vacutainer®Push Button Blood Collection Set（采血针）。
2.肢体向下放置。
3.握住采血管时塞子向上。
4.一旦血液开始流入管道，立即释放止血带。
5.静脉穿刺过程中，确保采血管内添加剂不会碰到塞子或针头末端。
建议抽血顺序
1. 无菌样本采血管
2. 无添加剂采血管
3. 用于凝集研究（如柠檬酸）的采血管
4. 含有其它冻干添加剂的采血管（如BD Vacutainer®，肝素管，EDTA管，BD P100血浆管或血清管）
5. 含有液体添加剂的采血管（如PAXgene® 采血管）
静脉穿刺技术和样本采集指导
1. 采用BDVacutainer®Safety-Lok™或BD Vacutainer® Push Button Blood Collection Set采集血液时，必需使用12英寸血液采集装置（采血针）。
2. 在静脉穿刺过程中和处理采血管时要戴手套，以尽量减少血液接触危险。
3. 选择所需样本的管子。
4. 将带有12英寸管的血液采集装置（采血针）组装到BD Vacutainer® One Use Holder（持针器）中。确保采血针牢固地连接到持针器上，并且在使用过程中不会松脱。
5. 将采血管放入持针器。注意：不要刺破塞子。
6. 选择静脉穿刺部位。
7. 使用止血带。静脉穿刺部位进行无菌消毒。消毒后请勿触诊静脉穿刺部位。
8. 将受检者的肢体置于向下的位置。
9. 拆下针头护套。肢体向下管塞向上进行静脉穿刺（参见防止回流部分）。
10. 在持针器中心放置采血管，以防止侧壁穿透并导致过早的真空损失。
11. 快速将采血管推到针头的非患者端（NP端），以尽量减少机械分离器与塞子的过早分离。在抽血过程中将管保持在NP端。
12. 如果采血过程中分离器在采血管中移动，请丢弃管并重新抽血。
13. 一旦血液出现在采血管中，立即取下止血带。在操作过程中，不要让管子的内容物接触塞子或针头。
14. 在从针头的NP（非患者）端拔出试管之前，要耗尽管内真空。
15. 将样本与试管添加剂混合。为了达到*混合效果，在离心分离前立即将采血管轻轻颠倒混匀8至10次。
离心
为了尽量减少血小板数量，离心条件参考推荐。
RCF（g） | 时间（分） |
1100 | 30 |
1600 | 30 |
2500 | 20 |
BD P100采血管在平衡离心机中可承受高达3000g的离心力。
相对离心力（g）与离心机转速设定（rpm）有关，用以下任一方程式：其中“r” 的单位是cm，表示的是从离心机头中心到管底部的径向距离。
rpm=√（g×105/1.12×r）或近似的rpm=10000/√r
BD Vacutainer® Hemogard™ 安全头盖的拆除说明
1. 用一只手抓住采血管，将拇指放在BD Hemogard™安全头盖下（为了更加稳固，可将手臂靠在坚硬的平面上）。另一只手扭转Hemogard头盖同时用另一只手的拇指往上提头盖。
直到塞子松动。
2.拔开之前移开拇指。不要使用大拇指推开盖子。
注意：任何试管都有破裂危险。如果试管含有血液，则存在血液接触危险。为了防止在头盖拆除过程中受到伤害，一旦Hemogard™安全头盖有松动，推头盖的大拇指就要移开，不要在接触管子。
3. 拔开头盖。如果塑料保护与胶塞分离，不要把头盖装回去。小心将塞子从试管中拔出。
保存
除非包装标签上另有说明，请将试管保存在4-25ºC（39-77ºF）。使用前建议将试管保存在原装密封包装中。包装带有干燥剂提供防潮层以保持添加剂的效力。
警告和注意事项
1.使用BDVacutainer®Safety-Lok™或BD Vacutainer®Push Button Blood Collection Set等采集血液时，一定要使用12英寸血液采集装置。
2.对眼睛、呼吸系统和皮肤有刺激性。如接触眼睛，立即用大量清水冲洗并寻求医疗指导。佩戴合适的手套和眼睛/脸部保护装置。
3.不要重复使用BD P100采血管。
4.由于试管管中含有化学添加剂（抗凝血剂和冻干添加剂），因此抽血过程中应采取措施以防止液体从管中回流至人体（请参阅防止回流部分并参阅MSDS了解化学预防措施）。
5.过度的离心速度（超过10000g）可能导致试管破裂，接触血液并可能造成伤害。
6.装有血液样本的试管储存在0℃或0℃以下时可能会导致试管破裂。
7.不要用拇指滚动打开安全头盖。通过扭转和拉动来移除头盖（参见BD Vacutainer® Hemogard™ 安全头盖的拆除说明）。
8.内毒素未被控制。BD P100采血管收集和处理血液和血液成分不适用于输液和引入人体。
注意：
1.练习标准注意事项。使用手套、长袍、护目镜、其他个人防护装备和工程控制装置，以防止血液飞溅，血液渗漏和潜在的血液传播病原体接触。
2.根据处理安全规范处理所有生物样本和采血用“尖锐物品”（柳叶刀、针头、鲁尔接头和血液采集器）。万一接触到生物样本（例如因穿刺而受伤），应采取适当的医疗措施，因为这些样本可能带有肝炎病毒，HIV或其他传染病。若采血器材具备安全装置，请善加利用。 BD不建议将用过的针头重新复帽。但是，您的设施的政策和程序可能有所不同，必须始终遵守。
3.采集血液用的任何“尖锐物品”应当丢进专门处置危险物品的容器中。
4.不建议使用注射器和针头将收集的样本转移到试管中。额外的“尖锐物品”操作，如空心针会增加针刺伤害的可能性。
5.出于下述原因，应谨慎使用非尖锐装置将样品从注射器转移到管中。 •在传输过程中按压注射器柱塞可能会产生正压，使塞子和样本移位，造成飞溅和潜在的血液接触。 •使用注射器进行血液转移也可能导致试管过量灌注或没有注满，导致血液与添加剂比率不正确，影响分析结果。 •BD P100采血管设计用来吸取标示体积，当真空耗净时，试管注满。但是当从注射器注血时，由于柱塞阻力，一些试管可能部分注满。使用这些样品时应该咨询实验室有关情况。
6.如果通过静脉（I.V.）管收集血液，在注采血管之前要确保管内I.V.溶液已清除。这对避免来自I.V.液体污染造成错误的实验室数据至关重要。
7.试管的过度灌装或没有注满会导致血液与添加剂的比例不正确，并可能导致分析结果不正确或产品性能不佳。
8.使用前，让BD P100 采血管达到室温。
BD P100采血管说明书中文版翻译：红荣微再编辑。请与英文原版说明书配合使用。
华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司代理BD采血管系列。BD P100 血浆管（货号：366448 采血量：8.5mL）现货*（2018年5月）。
BD P100英文原版说明书
BD P100
Blood Collection System
for Plasma Protein Preservation
For Research Use Only
Not For Use in Diagnostic Procedures
INTENDED USE
BD™ P100 is a Blood Collection System with a integrated mechanical separator that provides a means for collection, separation, transportation and preservation of plasma to be used in protein analysis. This product is for research use only and therefore the use of this product for diagnostic procedures and subject management is strictly prohibited. Neither the clinical utility nor the performance characteristics of the tube as part of an in vitro diagnostic procedure has been established.
PRODUCT DESCRIPTION
The BD P100 Tube contains spray-dried K2EDTA anticoagulant and proprietary additives.
During centrifugation, the separator device is activated and moves into position between the plasma and cellular elements. After separation, plasma may be aspirated directly from the collection tube, eliminating the need to transfer the plasma to a secondary tube.
BLOOD COLLECTION AND HANDLING
Required Equipment Not Provided
1. Blood Collection Set with 12 inch tubing such as the BD Vacutainer® Safety-Lok™ or
BD Vacutainer® Push Button Blood Collection Set.
2. A BD Vacutainer® Holder such as the BD Vacutainer® One Use Holder must be used to
ensure proper function.
3. Disposable transfer pipettes if direct sampling from the instrument is not used or if the
specimen is stored separay.
4. Centrifuge capable of generating 1100 – 3000 g at the bottom of the tube.
5. Gloves, eye protection and other personal protective equipment as necessary for
protection from exposure to blood borne pathogens.
Prevention of Backflow
The BD P100 blood collection tube contains chemical additives. It is important to avoid
possible backflow from the tube, with the possibility of adverse subject reaction. To guard
against backflow observe the following precautions:
1. Use a blood collection set such as the BD Vacutainer® Safety-Lok™ or BD Vacutainer®
Push Button Blood Collection Set.
2. Place limb in a downward position.
3. Hold tube with stopper upper-most.
4. Release tourniquet as soon as blood starts to flow into the tube.
5. Make sure that tube additives do not touch the stopper or the end of the needle during venipuncture.
Recommended Order of Draw
1. Tubes for sterile samples
2. Tubes without additives
3. Tubes for coagulation studies (e.g., citrate)
4. Tubes with other lyophilized additives (e.g., BD Vacutainer®, Heparin, EDTA, Plasma
BD P100, or Serum Tubes)
5. Tubes with other liquid additives (e.g. PAXgene® Tubes)
Venipuncture Technique and Specimen Collection General Instructions
1. It is absoluy essential to use a 12 inch blood collection set, when collecting blood using this device such as BD Vacutainer® Safety-Lok™ or BD Vacutainer® Push Button Blood Collection Set.
2. Wear gloves during venipuncture and when handling blood collection tubes to minimize exposure hazard.
3. Select tube or tubes for required specimen.
4. Assemble a blood collection set with 12 inch tubing into a BD Vacutainer® One Use Holder.
Be sure that blood collection set is firmly attached to holder and does not unthread during use.
5. Place tube into holder. Note: Do not puncture stopper.
6. Select site for venipuncture.
7. Apply tourniquet. Prepare venipuncture site with appropriate antiseptic. Do not palpate venipuncture site after cleansing.
8. Place subject’s limb in a downward position.
9. Remove needle shield. Perform venipuncture with limb downward and tube stopper up (refer to the Prevention of Backflow section).
10. Center tubes in the holder, to prevent sidewall penetration and resultant premature vacuum loss.
11. Push tube onto non-patient-end (NP-end) of needle in one swift action in order to minimize premature separation of the mechanical separator from the stopper. Hold tube on NP-end during drawing.
12. If separator moves in the tube during blood collection, discard the tube and redraw.
13. Remove tourniquet as soon as blood appears in tube. Do not allow contents of the tube to contact the stopper or end of the needle during procedure.
14. Allow vacuum to be exhausted prior to removing the tube from the NP (non-patient) end of the needle.
15. Mix specimen with tube additives. For optimal mixing,slowly invert tube 8 to 10 times immediay after blood collection before centrifugation.
Centrifugation
To achieve the lowest plaet count, refer to the recommended centrifugation conditions outlined in the following table.
The tube will withstand up to 3000 g in a balanced centrifuge.
Relative Centrifuge Force (g) is related to centrifuge speed setting (rpm) using either of the
following equations: where “r” expressed in cm, is the radial distance from the center of the
centrifuge head to the bottom of the tube.
Instructions for Removal of BD Vacutainer® Hemogard™ Closure
1. Grasp the BD P100 Tube with one hand, placing the thumb under the BD Hemogard™Closure. (For added stability, place limb on solid surface.) With the other hand, twist the Hemogard Closure while simultaneously pushing up with the thumb of the other hand
ONLY UNTIL THE TUBE STOPPER IS LOOSENED.
2. Move thumb away before lifting closure. DO NOT use thumb to push closure off the tube.
Caution: Any tube has the potential to crack. If the tube contains blood, an exposure hazard exists. To help prevent injury during removal, it is important that the thumb used to push upward on the closure be removed from contact with the tube as soon as the Hemogard™ Closure is loosened.
3. Lift closure off the tube. In the unlikely event of the plastic shield separating from the rubber stopper, DO NOT REASSEMBLE THE CLOSURE. Carefully remove stopper from the tube.
Storage
Store tubes at 4-25 ºC (39-77 ºF) unless otherwise noted on the package label. It is recommended to store the tubes in the original sealed packaging until use. The pouch with desiccant provides a moisture barrier to preserve the potency of the additives.
WARNINGS AND PRECAUTIONS
1. It is absoluy essential to use a 12 inch blood collection set, when collecting blood using these tubes such as BD Vacutainer® Safety-Lok™ or BD Vacutainer®Push Button Blood Collection Set.
2. Irritating to eyes, respiratory system and skin. In case of contact with eyes, rinse immediay with plenty of water and seek medical advice. Wear suitable gloves and eye/face protection.
3. Do not re-use the tube.
4. Since this tube contains chemical additives (anti-coagulant and a lyophilized additive) precautions should be taken to prevent possible backflow from the tube during blood drawing (see Prevention of Backflow section and refer to the MSDS for chemical precautions).
5. Excessive centrifugation speed (over 10,000 g) may cause tube breakage, exposure to blood and possible injury.
6. Storage of tubes containing blood at or below 0 ºC may result in breakage.
7. Do not remove closure by rolling with thumb. Remove with a twist and pull motion (see Instructions for Removal of the BD Hemogard™ Closure section).
8. Endotoxin not controlled. Blood and blood components collected and processed in the tube
are not intended for infusion or introduction into the human body.
CAUTION:
1. Practice Standard Precautions. Use gloves, gowns, eye protection, other personal protective equipment, and engineering controls to protect from blood splatter, blood leakage, and potential exposure to blood borne pathogens.
2. Handle all biologic samples and blood collection “sharps” (lancets, needles, luer adapters,and blood collection sets) according to the policies and procedures of your facility. Obtain appropriate medical attention in the event any exposure to biologic samples (for example, through a puncture injury), since they may transmit viral hepatitis, HIV (AIDS), or other infectious diseases. Utilize any built-in used needle protector, if the blood collection device provides one. BD does not recommend reshielding used needles. However, the policies and procedures of your facility may differ and must always be followed.
3. Discard all blood collection “sharps” in biohazard containers approved for their disposal.
4. Transferring a sample collected using syringe and needle to a tube is not recommended. Additional manipulation of sharps such as hollow bore needles increases the potential for needlestick injury.
5. Transferring samples from syringe to an tube using a non-sharps device should be performed with caution for the reasons described below. • Depressing the syringe plunger during transfer can create a positive pressure, forcefully displacing the stopper and sample,causing splatter and potential blood exposure. • Using a syringe for blood transfer may also cause over or underfilling of tubes, resulting in an incorrect blood-to-additive ratio and potentially incorrect analytic results. • Tubes are designed to draw the volume indicated. Filling is complete when vacuum no longer continues to draw, though some tubes may partially fill due to plunger resistance when filled from a syringe. The laboratory should be consulted regarding the use of these samples.
6. If blood is collected through an intravenous (I.V.) line, ensure that line has been cleared of I.V. solution before beginning to fill blood collection tubes. This is critical to avoid erroneous laboratory data from I.V. fluid contamination.
7. Overfilling or underfilling of tubes will result in incorrect blood-to-additive ratio and may lead to incorrect analytic results or poor product performance.
8. Allow the BD P100 Tube to come to room temperature before use.
华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司代理BD采血管系列。BD P100 血浆管（货号：366448 采血量：8.5mL）现货*（2018年5月）。
