BioGems重组人转铁蛋白是在无动物源的GMP车间生产的,活性相当于人血清铁饱和的转铁蛋白(holo transferrin),纯度≥ 95% 。适用于细胞培养。
BioGems重组人转铁蛋白参数
别名 Serotransferrin(血清转铁蛋白), TF(转铁蛋白), TFQTL1, PRO2086, PRO1557
表达系统 Oryza Sativa 水稻
氨基酸序列 VPDKTVRWC AVSEHEATK CQSFRDHMK SVIPSDGPS VACVKKASY LDCIRAIAA NEADAVTLD AGLVYDAYL APNNLKPVV AEFYGSKED PQTFYYAVA VVKKDSGFQ MNQLRGKKS CHTGLGRSA GWNIPIGLL YCDLPEPRK PLEKAVANF FSGSCAPCA DGTDFPQLC QLCPGCGCS TLNQYFGYS GAFKCLKDG AGDVAFVKH STIFENLAN KADRDQYEL LCLDNTRKP VDEYKDCHL AQVPSHTVV ARSMGGKED LIWELLNQA QEHFGKDKS KEFQLFSSP HGKDLLFKD SAHGFLKVP PRMDAKMYL GYEYVTAIR NLREGTCPE APTDECKPV KWCALSHHE RLKCDEWSV NSVGKIECV SAETTEDCI AKIMNGEAD AMSLDGGFV YIAGKCGLV PVLAENYNK SDNCEDTPE AGYFAIAVV KKSASDLTW DNLKGKKSC HTAVGRTAG WNIPMGLLY NKINHCRFD EFFSEGCAP GSKKDSSLC KLCMGSGLN LCEPNNKEG YYGYTGAFR CLVEKGDVA FVKHQTVPQ NTGGKNPDP WAKNLNEKD YELLCLDGT RKPVEEYAN CHLARAPNH AVVTRKDKE ACVHKILRQHLFGSNV TDCSGNFCL FRSETKDLL FRDDTVCLA KLHDRNTYE KYLGEEYVK AVGNLRKCS TSSLLEACT FRRP
质检 通过N端和质谱分析进行验证(适用时)。Verified by N-terminal and Mass Spectrometry analyses(when applicable).
纯度 ≥ 95% by SDS-PAGE.
内毒素水平 Endotoxin level is < 1 EU/mg.
生物活性 在无血清添加培养基中通过与天然人转铁蛋白在杂交瘤细胞的抗体生产中比较来证实生物学活性。Biological activity verified by comparison against native human transferrin in the antibody production of hybridoma cells in serum-free supplemented media.
处理 0.2μm灭菌过滤。从磷酸钠和氯化钠溶液(pH7.0)冻干。