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液体活检:外周血游离循环肿瘤细胞(CTC)保存管
更新时间:2018-01-26   点击次数:4869次

肿瘤CTC介绍

Clinical Applications of Circulating Tumor Cells and Circulating Tumor DNA as Liquid Biopsy,

Catherine Alix-Panabières,Cancer Discov;6(5); 479–91. ©2016 AACR Published OnlineFirst March 11, 2016; DOI: 10.1158/2159-8290.CD-15-1483.

 

原发肿瘤(乳房、结肠、肺、前列腺癌)在形成生长早期,会释放细胞进入血液循环。通过各种技术可以富集检测这些循环肿瘤细胞的物理和生物性质。

循环肿瘤细胞,Circulating Tumor cell,简称CTC。CTC释放点:原发肿瘤和转移肿瘤。

CTC死亡后还会释放出循环肿瘤DNA,Circulating Tumor DNA,简称ctDNA

 

CTC和ctDNA的临床应用

肿瘤检测 CTCs和ctDNA应用肿瘤的早期检测很受关注,面临的挑战是目前分析的特定性和敏感度。

预后评估 多种肿瘤已经实现预后评估,尤其是乳癌。

指导系统治疗

病人亚群分类(stratification)。

 

 

外周血游离循环肿瘤细胞(CTC)保存管

——Streck Cell-Free DNA BCT® 血浆游离DNA采血/保存管内含特殊保护剂,可以保护循环肿瘤细胞,常温下可以保存CTC达7天。

英文名称:Streck Cell-Free DNA BCT® BLOOD COLLECTION TUBE

中文名称:Streck BCT® 血浆游离DNA采血/保存

货号:218962,230253

 

Streck 血浆游离DNA采血管是一种直接提取的全血收集管,用于收集、运输和储存血液样本。Streck 血浆游离DNA采血管内含防腐剂,它能稳定有核血细胞,防止细胞基因组DNA的释放,保护血浆游离循环DNA和循环肿瘤细胞(CTC)。

230253是Streck Cell-Free DNA BCT® 10mL采血管218962的升级版,是Streck公司新推将玻璃和塑料的*性能融合在一起的Fusion2TM混合塑料管

 

Streck Cell-Free DNA BCT® 血浆游离DNA采血/保存管应用

NIPT无创产前检测:孕妇外周血的胎儿游离DNA保存

肿瘤液体活检:循环肿瘤细胞(CTCs)和ctDNA(循环肿瘤DNA)保存

其他:血浆基因组DNA和游离DNA保存

 

Streck Cell-Free DNA BCT® 血浆游离DNA采血/保存管基因保存性能

游离DNA、细胞内基因组DNA和循环肿瘤细胞样本保存/稳定性

 

样本类型

游离DNA

胞内基因组DNA

肿瘤细胞(上皮细胞)

样本保存时间

14天

14天

7天

样本保存温度

6-37℃

6-37℃

15-30℃

 

 

Streck 218962(现货),230253 Cell-Free DNA BCT采血管欢迎您咨询华雅再生医学旗舰公司:红荣微再(上海)生物工程技术有限公司  :1500 1904 520。红荣微再-客服: 2395557778  经销商专员

 

 

 

 

外周血游离循环肿瘤细胞(CTC)保存管

——Streck Cell-Free DNA BCT® 血浆游离DNA采血/保存管

文献使用Circulating Tumor Cells (CTCs)

Cellular expression of PD-L1 in the peripheral blood of lung cancer patients is associated with worse survival

Boffa D.J., Graf R.P., Salazar M.C., Hoag J., Lu D., Krupa R., et al.

Cancer Epidemiology, Biomarkers & Prevention, 2017.

Chromosomal instability estimation based on next generation sequencing and single cell genome wide copy number variation analysis

Greene S.B., Dago A.E., Leitz L.J., Wang Y., Lee J., Werner S.L., et al.

PLoS One, 2016.

Development of an automated and sensitive microfluidic device for capturing and characterizing circulating tumor cells (CTCs) from clinical blood samples

Gogoi P., Sepehri S., Zhou Y., Gorin M.A., Paolillo C., Capoluongo E., et al.

PLoS One, 2016.

Detection and characterization of circulating tumour cells in multiple myeloma

Zhang L., Beasley S., Prigozhina N.L., Higgins R., Ikeda S., Lee F., et al.

Journal of Circulating Biomarkers, 2016.

Association of AR-V7 on circulating tumor cells as a treatment-specific biomarker with outcomes and survival in castration-resistant prostate cancer

Scher H.I., Lu D., Schreiber N.A., Louw J., Graf R., Vragas H.A., et al.

Journal of the American Medical Association Oncology, 2016.

A novel approach for next-generation sequencing of circulating tumor cells

Yee S.S., Lieberman D.B., Blanchard T., Rader J., Zhao J., Troxel, A.B., et al.

Molecular Genetics & Genomic Medicine, 2016.

Analytical validation and capabilities of the Epic CTC platform: enrichment-free circulating tumour cell detection and characterization

Werner S.L., Graf R.P., Landers M., Valenta D.T., Schroeder M., Greene S.B., et al.

Journal of Circulating Biomarkers, 2015.

Fluid phase biopsy for detection and characterization of circulating endothelial cells in myocardial infarction

Bethel K., Luttgen M.S., Damani S., Kolatkar A., Lamy R., Sabouri-Ghomi M., et al.

Physical Biology, 2014.

Clinical evaluation of a novel microfluidic device for epitope-independent enrichment of circulating tumour cells in patients with small cell lung cancer

Chudziak J., Burt D.J., Mohan S., Rothwell D.G., Mesquita B., Antonello J., et al.

Analyst, 2015.

Detection and characterization of circulating tumour cells from frozen peripheral blood mononuclear cells

Lu D., Graf R.P., Harvey M., Madan R.A., Heery C., Marte J., et al.

Journal of Circulating Biomarkers, 2015.

Limited genomic heterogeneity of circulating melanoma cells in advanced stage patients

Ruiz C., Li J., Luttgen M.S., Kolatkar A., Kendall J.T., Flores E., et al.

Physical Biology, 2015.

PTEN loss in circulating tumour cells correlates with PTEN loss in fresh tumour tissue from castration-resistant prostate cancer patients

Punnoose E.A., Ferraldeschi R., Szafer-Glusman E., Tucker E.K., Mohan S., Flohr P., et al.

British Journal of Cancer, 2015.

Rapid changes in circulating tumor cells following anti-angiogenic therapy

Gross M.E., Dorff T.B., Quinn D.I., Agus D.B., Luttgen M., Bethel K., et al.

Convergent Science Physcial Oncology, 2015.

Circulating tumor microemboli diagnostics for patients with non-small-cell lung cancer

Carlsson A., Nair V.S., Luttgen M.S., Keu K.V., Horng G., Vasanawala M., et al.

Journal of Thoracic Oncology, 2014.

An observational study of circulating tumor cells and 18F-FDG PET uptake in patients with treatment-naïve non-small cell lung cancer

Nair V.S., Keu K.V., Luttgen M.S., Kolatkar A., Vasanawala M., Kuscher W., et al.

PLoS One, 2013.

Optical quantification of cellular mass, volume, and density of circulating tumor cells identified in an ovarian cancer patient

Phillips K.G., Velasco C.R., Li J., Kolatkar A., Luttgen M., Bethel K., et al.

Frontiers in Oncology, 2012.

Quantification of cellular volume and sub-cellular density fluctuations: comparison of normal peripheral blood cells and circulating tumor cells identified in a breast cancer patient

Phillips K.G., Kolatkar A., Rees K.J., Rigg R., Marrinucci D., Luttgen M., et al.

Frontiers in Oncology, 2012.

Multiple biomarker expression on circulating tumor cells in comparison to tumor tissues from primary and metastatic sites in patients with locally advanced/ inflammatory, and stage IV breast cancer, using a novel detection technology

Somlo G., Lau S.K., Frankel P., Hsieh H.B., Liu X., Yang L., et al. Breast Cancer Research and Treatment, 2011.

Cytomorphology of circulating colorectal tumor cells: a small case series

Marrinucci D., Bethel K., Lazar D., Fisher J., Huynh E., Clark P., et al.

Journal of Oncology, 2010.

Sensitive characterization of circulating tumor cells for improving therapy selection

Hsieh H.B., Somlo G., Bennis R., Frankel P., Krivacic R.T., Lau S., et al.

SBEC, 2010, IFMBE Proceedings 32.

外周血游离循环肿瘤细胞(CTC)保存管

——Streck Cell-Free DNA BCT® 血浆游离DNA采血/保存管

 

 

 

订购信息

 

品牌

货号

产品描述

包装

Streck

218962

Cell-Free DNA BCT® BLOOD COLLECTION TUBE

Cell-Free DNA BCT®血浆游离DNA采血管

100管/盒

Streck

230253

Cell-Free DNA BCT® BLOOD COLLECTION TUBE

Cell-Free DNA BCT®血浆游离DNA采血管(混合塑料)

100管/盒

 

外周血游离循环肿瘤细胞(CTC)保存管

Streck 218962(现货),230253 Cell-Free DNA BCT采血管及更多液体活检产品欢迎您咨询华雅再生医学旗舰公司:红荣微再(上海)生物工程技术有限公司  :1500 1904 520。红荣微再-客服: 2395557778  经销商专员红荣微再以“传递科学价值,服务科学研究”为宗旨主营干细胞、医疗、细胞治疗、器官再生四大板块的产品

 

 

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