产品描述

ScienCell 1001 ECM内皮细胞培养基是经灭菌的液体培养基，包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子（ECGS\ECGF）、微量矿物质和低浓度胎牛血清(5%)。内皮细胞培养基（ECM）缓冲体系为重碳酸盐，在含5%CO2的细胞培养箱中平衡后pH值为7.4。内皮细胞培养基（ECM）的配方能够选择性的促进正常人类微血管内皮细胞体外培养中的增殖和生长，并为其达到营养平衡状态提供数量上和质量上的保证。

产品组份

500mL基础培养基，25mL FBS，5mL内皮细胞生长因子（ECGS），5mL penicillin/streptomycin solution。

注意：FBS, ECGS 和P/S没有预混好。要配制完整的ECM培养基需要分别添加。 

使用

Sciencell 1001 内皮细胞培养基仅供科研使用。

储存

基础培养基：4℃。ECGS, FBS and P/S 溶液：-20℃。避光。

运输

基础培养基：室温。添加剂：干冰。

使用说明：

ECGS, FBS and P/S 溶液37℃溶解。加入培养基前试管弹拨几次使内容物充分溶解。培养基瓶子和试管用70%酒精消毒。无菌环境中，开盖时注意不要让手指碰到内壁。添加ECGS, FBS and P/S 到培养基并且充分混匀。由于多种成分是光不稳定的，培养基要避光使用。使用前不推荐用37℃水浴加热培养基。避光4℃保存时，重新配制的培养基能稳定放置1个月。

注意：如果没有正确使用，培养基的某些成分会影响健康。处理时做好防护措施，比如穿防护衣和防护镜。

——————————————————————————————————————

英文原版

Product Description

Endothelial Cell Medium (ECM), when used with Endothelial Cell Growth Supplement (ECGS,

Cat #1052) and 25 ml of fetal bovine serum (FBS) is a complete medium designed for optimal

growth of normal endothelial cells in vitro. It is a sterile, liquid medium which contains essential

and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth

factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is

HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an

atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to

provide an optimally balanced nutritional environment that selectively supports the growth of

normal endothelial cells in vitro.

Components

ECM consists of 500 ml of basal medium, 25 ml of FBS (Cat. #0025), 5 ml of Endothelial Cell

Growth Supplement (ECGS, Cat. #1052) and 5 ml of penicillin/streptomycin solution (P/S, Cat.

#0503). Note: FBS, ECGS and P/S are not pre-mixed in ECM; they must be added separay to

make the complete ECM.

Product Use

ECM is for research use only. It is not approved for human or animal use, or for application in in

vitro diagnostic procedures.

Storage

Store the basal medium at 4oC and the ECGS, FBS and P/S solution at -20oC. Protect from light.

Shipping

Basal medium: room temperature. Supplements: dry ice.

Instructions for Use

Thaw ECGS, FBS and P/S solution at 37oC. Gently tilt the tubes several times to ensure the contents are compley mixed before adding to the medium. Spray the medium bottle and tubes with 70% ethanol, and wipe to remove excess liquid. In a sterile field, remove the caps without touching the interior threads with fingers. Add ECGS, FBS and P/S solution to the medium and mix well. Since several components are light-labile, the medium should not be exposed to light for extended periods. We do not recommend warming medium in a 37oC water bath prior to use.When stored in the dark at 4oC, the reconstituted medium is stable for one month.

Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.

ScienCell ECM 1001内皮细胞培养基说明书中文版由红荣微再编辑翻译。

ScienCell ECM 1001内皮细胞培养基订购信息

欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司  ：1500 1904 520。红荣微再-客服: 2395557778  经销商专员