BD Vacutainer CPT单个核细胞制备管提供了一种快速分离浓缩血小板（PRP）的方法，一次离心（zui多半小时），即可得到PRP。可以作为PRP美容采血管。
BD Vacutainer CPT 采血管
产品订购信息
品牌 | 货号 | 产品描述 | 包装 |
BD | 362761 | Vacutainer® CPTTM Mononuclear Cell Preparation Tube 8.0mL单个核细胞准备管，0.1M的柠檬酸钠1.0mL，浅蓝/黑色盖 特别应用：PRP采血管 | 60支/箱 |
———————————————BD 362761现货直达————————————————
PRP美容采血管自体血清制备BD Vacutainer CPT Mononuclear Cell Preparation Tube 362761单个核细胞准备管
欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司 ：1500 1904 520。红荣微再-客服: 2395557778 经销商专员
BD 362761采血管参数
REF 362761
8 mL Draw Capacity (16 x 125 mm Tube Size) Sterile Tube Interior
表面材质
Silicone Coated Glass Tube
Silicone Lubricated Rubber Stopper
密度梯度原理：FICOLL法
抗凝剂1.0 mL of 0.1 Molar Sodium Citrate Solution (Top Fluid Layer)
分离胶
3.0 gm of Polyester Gel (Middle Layer)
2.0 mL of Polysaccharide/Sodium Diatrizoate Solution (FICOLL™ Hypaque™ solution, Bottom Fluid Layer)
离心 20 minutes at 1500 to 1800 RCF。zui高30min。
分析
Recovery and Purity percentages：
Coulter Counter® Model S PLUS IV
Viability percentages：
Acridine Orange/Ethidium Bromide staining
Red blood cell percentages：
Hemocytometer count under a light microscope
BD Vacutainer CPT采血管系列参考资料：
GENERAL REFERENCES
Centers for Disease Control. Recommendations for Prevention of HIV Transmission in Health-Care Settings. MMWR 1987, 36 (suppl.no. 2S), pp. 3S - 17S.
Centers for Disease Control. Update: Universal Precautions for Prevention of Transmission of Human Immunodeficiency Virus,Hepatitis B Virus, and Other Bloodborne Pathogens in Health-Care Settings. MMWR 1988, 37 No. 24, pg. 380.
National Committee for Clinical Laboratory Standards. Protection of Laboratory Workers from Infectious Disease Transmitted by Blood Tissue. Tentative Guidelines NCCLS Document M29-T.NCCLS; 1991, Villanova, PA.
OSHA Final Standard for Occupational Exposure to Bloodborne Pathogens. 56 Fed. Reg. 64 175, Dec. 6, 1991; 29 CFR Part 1910.1030.
REFERENCES
1. Boyum, A. Isolation of mononuclear cells and granulocytes from human blood. Scand. J.Clin. Lab. Invest. 21, Suppl 97 (Paper IV), 77-89, 1968.
单个核细胞分离
2. Ting, A. and Morris, P.J. A technique for lymphocyte preparation from stored heparinized blood. Vox. Sang. 20:561-563,1971.
肝素血制备淋巴细胞
3. Fotino, M., Merson, E.J. and Allen, F.H. Micromethod for rapid separation of lymphocytes from peripheral blood. Ann. Clin. Lab. Sci. 1:131-133, 1971.
淋巴细胞快速分离
4. Dwyer, J.M., Finklestein, F.O., Mangi, R.J., Fisher, K. and Hendler, E. Assessment of the adequacy of immunosuppressive therapy using microscopy techniques to study immunologic competence. Transplant. Proc. 7:785, 1975.
5. Brown, G. and Greaves, M.F. Enumeration of absolute numbers of T and B cells in human blood. Scand. J. Immunol. 3:161, 1974.
T细胞和B细胞计数
6. McCarthy, D.A., Perry, J.D., et.al. Centrifugation of Normal and Rheumatoid Arthritis Blood on FICOLL™-Hypaque™ and FICOLL™-Nycodenz Solution. J. Immunol. Meth. 73:415-425 (1984).
分离液FICOLL的离心
7. Data on File, Report No. R-88-99-QC-195, BD Vacutainer Systems, NJ
8. Needham, P.L. Separation of human blood using "Mono- Poly Resolving Medium." J. Immunol. Meth. 99:283, 1987
PRP介绍
PRP为Plaet-rich plasma的缩写，中文为“高浓度血小板血浆”，PRP具有迅速止血、止痛、加速伤口愈合的作用。PRP的意思是富血小板血浆，PRP血清因子更是明星永葆青春的秘密，PRP自体血清注射回春术用自身血液制作出富含高浓度血小板和自身生长因子的血浆，重新注射到组织中，PRP自体血清注射回春术提高皮肤内胶原蛋白和弹力纤维的含量。利用少量自身血液用高科技分离技术，使用BD362761/362760/362753系列PRP采血管，可分离出含高浓度细胞生长因子的富血小板血清“Plaet-Rich Plasma”，简称PRP。然后再注入肌肤，其活性PRP内里蕴藏多种皮层生长及修复因子，能修补衰老受损肌肤，激活皮肤细胞功能，改善新陈代谢，让肌肤注满能量并得以再生，延缓衰老。接受ACR-PRP激活肌肤疗程后，一般于数周至一个月骨可见肌肤质素有多方面的改善，包括弹性、光泽度、紧致度和嫩滑程度等，让肌肤焕发青春神采。
———————————————BD 362761采血管现货直达—————————————
PRP美容采血管自体血清制备BD Vacutainer CPT Mononuclear Cell Preparation Tube 362761单个核细胞准备管
欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司 ：1500 1904 520。红荣微再-客服: 2395557778 经销商专员
