1.推荐加样量

1-10个人类细胞（如：卵裂球，极体，滋胚层细胞，羊水细胞，循环肿瘤细胞，培养细胞）

1000-10000个细菌细胞

不完整或片段化，单/双链DNA

2.PicoPLEX® 单细胞全基因组扩增试剂盒推荐应用？

PicoPLEX单细胞全基因组扩增试剂盒用于：

CNV拷贝数变异：寡核苷酸和细菌人工染色体的微阵列比较基因组杂交（oligonucleotide and BAC aCGHa）

单核苷酸多态性分型SNP

PCR法基因突变筛查

3.使用PicoPLEX WGA Kit前，可以用什么缓冲液洗涤单细胞？

一些客户成功使用加了PVA（聚乙烯醇，例：0.1%）的PBS洗涤收集细胞。另外，注意洗涤/收集缓冲液（PBS）不要含钙、镁离子。

4.流式分选有什么特殊要求吗？

Yes, we strongly recommend not fixing, and using light scattering or phase contrast to sort or collect samples. Microscopic/visual confirmation of successfully sorted cells can be used to optimize sorting conditions.

5.PicoPLEX WGA Kit的加样体积？

每次反应5 μl 加样体积（细胞和缓冲液总体积不超过2.5 μl）。

6.单细胞全基因组扩增产物能直接用于NGS吗？

不能。

3.那么有其他能直接应用NGS的单细胞全基因组扩增试剂盒吗？

有。PicoPLEX DNA-seq直接应用Illumina NGS。

8.What is the expected genomic locus representation and Loss of Heterozygosity (LOH) for PicoPLEX WGA products?

For reference purposes, see the data obtained from a third party in the table below.

9.单细胞全基因组扩增技术的终产物是单链还是双链DNA？

产物是单双链DNA的混合物。

PicoPLEX WGA Kit 单细胞全基因组扩增试剂盒问答英文版

1. What are the recommended DNA inputs for the PicoPLEX WGA Kit?

1 – 10 human cells (e.g. blastomeres, polar bodies, trophoblastic cells, amniocytes, CTCs, cultured cells

1,000 – 10,000 bacterial cells

Intact or fragmented, single- or double-stranded DNA

2.What applications are recommended for PicoPLEX® WGA Kit?

This kit is used for:

Copy number variation (CNV) analysis: superior for oligonucleotide and BAC aCGH（Bacterial Artificial Chromosome Array-based comparative genomic hybridization）

SNP genotyping

Mutation detection by PCR

3.  Is there a non-stick wash buffer that can be used for single cell wash prior to using PicoPLEX WGA Kit?

This has not been validated at Rubicon Genomics, but some customers have successfully used PVA (Polyvinyl Alcohol; e.g. 0.1%) in PBS to wash/collect their cells. Additionally, your washing/collecting buffer (PBS) must be also Ca- and Mg-free.

4.  Are there special requirements for flow sorting?

Yes, we strongly recommend not fixing, and using light scattering or phase contrast to sort or collect samples. Microscopic/visual confirmation of successfully sorted cells can be used to optimize sorting conditions.

5.  What is the sample input volume for PicoPLEX WGA Kit?

This kit will accommodate a 5 μl sample input volume per reaction (with a maximum of 2.5 μl of cell & buffer carryover).

6.  Can the product of PicoPLEX WGA Kit be taken straight to NGS?

No.

7.  Does Rubicon Genomics have an NGS-ready kit for single cell applications?

Yes, PicoPLEX DNA-seq can be used with Illumina NGS platforms.

8.  Sigma-Aldrich sells a GenomePlex WGA kit, is it the same technology as PicoPLEX WGA? 

No, PicoPLEX uses different technology.

9.  What is the expected genomic locus representation and Loss of Heterozygosity (LOH) for PicoPLEX WGA products?

For reference purposes, see the data obtained from a third party in the table below.

10.  Is the product of this technology single- or double-stranded?

The end product of PicoPLEX WGA is a mixture of single- and double-stranded DNA.

PicoPLEX WGA Kit 单细胞全基因组扩增试剂盒问答英文版摘自Rubicon，中文版由红荣微再翻译整理。

>>>>>>>>>>>>>>>>>>>>>>>>>>订购直达的分割线>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

选购RB3050 Rubicon PicoPLEX WGA Kit 单细胞全基因组扩增试剂盒产品欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司 ：152 1681 4001。华雅再生医学-客服: 316 808 6348。

RB3050 Rubicon PicoPLEX WGA Kit 单细胞全基因组扩增试剂盒

PicoPLEX WGA Kit采用技术，设计优化用于从单细胞中扩增全基因组DNA。起始样本量低至单细胞或6pg基因组DNA。便捷的单管操作流程降低污染节省时间。结果稳定性、重复性好。

PicoPLEX WGA Kit适用array CGH分析和PCR平台。PicoPLEX技术被IVF供应商如BlueGnome信赖并用于PGS，BlueGnome CGH也是中国福利会和平妇幼保健院所用的PGS平台。

产品订购信息

红荣微再（上海）生物工程技术有限公司以“传递科学价值，服务科学研究”为宗旨，主营：干细胞、医疗、细胞治疗、器官再生 四大板块的产品。

红荣微再医疗宣传册

选购RB3050 Rubicon PicoPLEX WGA Kit 单细胞全基因组扩增试剂盒产品欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司 ：152 1681 4001。华雅再生医学-客服: 316 808 6348。