AB血清对人体外终末期红系分化进程的影响

摘要

目的：探究体外诱导造血干细胞向红系分化过程中人AB血清对终末期红系分化进程的影响。

方法：从脐带血中用免疫磁珠法分离提取CD34+细胞，体外诱导其向红系分化。根据培养体系中是否添加人AB血清，设置实验组为添加人AB血清组，对照组为不添加人AB血清组。通过检测细胞表面红系分化标志分子糖化血红蛋白A(glycophorin A,GPA)、人红细胞阴离子交换蛋白Band3及整合素α4(α4-integrin)的表达反映终末红系分化进程,用流式细胞术检测红系细胞终末分化脱核情况。结果:与对照组相比,实验组GPA阳性细胞百分率较高,Band3表达较高,而α4-integrin表达较低(P<0.05)。实验组细胞脱核百分率明显高于对照组(P<0.05)。结论:在体外红系发育培养体系中,人AB血清对终末期红系分化进程有一定的促分化、促脱核作用。

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2016 Dec 28;41(12):1245-1251. doi: 10.11817/j.issn.1672-7347.2016.12.001.

[Effect of AB serum on human terminal erythroid differentiation ex vivo].

[Article in Chinese; Abstract available in Chinese from the publisher]

Liao J1,et al.

Abstract in English, Chinese

OBJECTIVE:

To demonstrate the effect of AB serum on terminal erythroid differentiation ex vivo.
 Methods: After separation of CD34+ cells from cord blood, the cells were cultured and divided into a control group and an experimental group. The effects of AB serum were examined by the expressions of different markers (GPA, Band3 and α4-integrin) for erythroblast differentiation and enucleation by flow cytometry. Results: The CD34+ cells were successfully differentiated to enucleated red blood cells. There were evident differences among the expressions of GPA, Band3 and α4-integrin between the 2 groups. The percentage of GPA positive cells in the experimental group was bigger than that in the control group in every time point. The expression of Band3 in the experimental group was higher than that in the control group. The expression of α4-integrin in the experimental group was lower than that in the control group. In addition, the enucleation rate in the experimental group was higher than that in the control group.
 Conclusion: AB serum can promote the cell differentiation and enucleation during terminal erythroid differentiation in vitro.

Free full text

原版中文

>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

欢迎您致电咨询Human Serum AB人AB血清 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司 ：152 1681 4001。华雅再生医学-客服: 316 808 6348

GemCell™人AB血清来自于美国FDA认证的AB血型健康男性供体。供体AB血浆加抗凝剂经去纤维蛋白处理得到AB血清，一致性较高。所有供体的病毒标志物检测都是阴性。

人AB血清作为牛血清（FBS、FCS）的替代物，不含外源物，适合组织工程、移植和细胞治疗领域的间充质干细胞扩增，及其他免疫系统相关的细胞培养和反应。

产品订购信息

红荣微再（上海）生物工程技术有限公司以“传递科学价值，服务科学研究”为宗旨，主营：干细胞、医疗、细胞治疗、器官再生四大板块的产品。