Characterization of Human AB Serum for Mesenchymal Stromal Cell Expansion
背景：
目前，使用人血源成分仿佛成为间充质干细胞（mesenchymal stromal cell，MSC）扩增zui有效zui安全的方法。这篇文献介绍了不同血浆来源的人AB血清的特征，以及人AB血清作为MSC扩增的补体使用。
方法：
人AB血清的两种血浆来源：全血采集后24h的血浆和去冷沉淀血浆（Cryoprecipitate-reduced plasma）。分析人AB血清不同批次的生化性质，并且评估它们储存不同时间后（0，3，6，9个月）对MSC细胞生长的影响。
结果：
两种血浆来源的人AB血清的生化组成和质量参数类似，而且都能有效促进MSC生长。添加了10%人AB血清的培养基和添加10%胎牛血清（fetal bovine serum，FBS）的培养基相比，MSC的doubling times and cumulative population doublings类似，同时MSC保持了免疫表型，功能特征和细胞遗传学特征。
结论：
总之，结果说明人AB血清是有效的FBS替代物，而且生产后能至少使用12个月，也不影响细胞增殖和质量。
Characterization of Human AB Serum for Mesenchymal Stromal Cell Expansion.
Transfus Med Hemother. 2017 Jan;44(1):11-21. doi: 10.1159/000448196. Epub 2016 Nov 2.
Dos Santos VT1,et al.
Abstract
BACKGROUND:
So far, using human blood-derived components appears to be the most efficient and safest approach available for mesenchymal stromal cell (MSC) expansion. In this paper, we report on the characterization of human AB serum (AB HS) produced by using different plasma sources, and its use as an alternative supplement to MSC expansion.
METHODS:
Two plasma sources were used for AB HS production: plasma removed from whole blood after 24 h of collection (PC > 24 h) and plasma, cryoprecipitate reduced (PCryoR). The biochemical profile and quality of the produced AB HS batches were analyzed and their ability to support MSC cell growth after different storage times (0, 3, 6, 9 and 12 months) was evaluated.
RESULTS:
The two plasma sources used showed similar characteristics regarding biochemical constituents and quality parameters and were effective in promoting MSC growth. MSCs cultured in medium supplemented with 10% AB HS presented similar doubling times and cumulative population doublings when compared to the 10% fetal bovine serum(FBS)-supplemented culture while maintaining immunophenotype, functional features, and cytogenetic profile.
CONCLUSION:
Overall, the results indicate that AB HS is an efficient FBS substitute and can be used for at least 12 months after production without impairing cell proliferation and quality.
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摘要翻译
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GemCell™人AB血清来自于美国FDA认证的AB血型健康男性供体。供体AB血浆加抗凝剂经去纤维蛋白处理得到AB血清，一致性较高。所有供体的病毒标志物检测都是阴性。
人AB血清作为牛血清（FBS、FCS）的替代物，不含外源物，适合组织工程、移植和细胞治疗领域的间充质干细胞扩增，及其他免疫系统相关的细胞培养和反应。
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GemCell | 100-512 | U.S. Origin Human Serum AB人AB血清 | 100 mL/瓶 |
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